Fumagilina mikotoxinak aspergilosi inbaditzailearen garapenean duen rola aztertzeko SPE-UHPLC-DAD metodo analitikoa
- Ane Yaldebere 1
- Oskar González-Mendia 1
- Xabier Guruceaga 1
- Andoni Ramirez-García 1
- Aitor Rementeria 1
- Rosa Alonso 1
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1
Universidad Politécnica de Valencia
info
ISSN: 0214-9001
Year of publication: 2023
Issue: 43
Pages: 41-58
Type: Article
More publications in: Ekaia: Euskal Herriko Unibertsitateko zientzi eta teknologi aldizkaria
Abstract
Invasive aspergillosis caused by Aspergillus fumigatus is a threat for immunocompromised patients. According to recent studies, fumagillin, a mycotoxin produced by this fungus, has been associated with the propagation of the disease. Therefore, this molecule might help to understand the mechanisms of this disease and to study the use of fumagillin as a potential biomarker of invasive aspergillosis. In spite of the relevance of fumagillin analysis in microbiological research, no quanti-tative method has been developed so far for its determination in cell culture media. Here, we present the first validated method for the quantitative analysis of fumagillin in RPMI-1640. The sample treatment consists of a mixed-mode anion exchange Solid Phase Extraction that effectively removes potential interferences and offered a recovery of 83 ± 7%. The analysis was carried out by Ultra High Performance Liquid Chromatography coupled to Diode Array Detection at 336 nm. The method fulfilled all the validation criteria established by EMA (European Medicine Agency) and FDA (Food and Drug Administration) guidelines for bioanalysis. Finally, the method was satisfactorily applied to the quantification of the fumagillin produced by different strains of Aspergillus fumigatus and it was observed that they had a different micotoxin production capacity.