NMR and molecular recognitionthe interaction of human galectin-4 with the histo blood group antigens and with pathogen-associated molecules

  1. QUINTANA GARCÍA, JON IMANOL
Supervised by:
  1. Jesús Jiménez Barbero Director
  2. Ana Ardá Freire Director
  3. María Nuria Sotomayor Anduiza Tutor

Defence university: Universidad del País Vasco - Euskal Herriko Unibertsitatea

Fecha de defensa: 05 October 2022

Department:
  1. Química Orgánica e Inorgánica

Type: Thesis

Teseo: 772305 DIALNET lock_openADDI editor

Abstract

Glycans are essential molecules that are ubiquitously found in every living cell. The interaction betweengalect in 4, a human sugar binding protein, with the histo blood group antigens has been studied by NuclearMagnetic Resonance ( Isothermal Titration Calorimetry and Molecular Dynamics simulations. Theeffect of the chemical modification of these antigens on the interaction with galectin 4 has been addressed.These antigens are mimicked by various pathogens, thus, the interaction between galectin 4 and theantigenic fragment of these pathogens ( LPS) has also been studied using NMR. Them ain co receptor for LPS, the glycoprotein CD14 has also been analysed. The relevance of each of theglycans of CD14 in its stability and folding, as well as for the interaction with galectin 4 has beencharacterized. Finally, using a solid phase synthesize r, the synthesis of oligosaccharides has beenperformed.