Evaluación del sistema maldi-tof vitek mstm para la identificación rápida de microorganismos de interés clínico

Abstract

The identification of microorganisms has been one of the main tasks of the microbiologist over time. Mass spectrometry (MS) has entered laboratories strongly, in principle, for the identification of microorganisms. The aim of the thesis was to demonstrate that mass spectrometry MALDI-TOF is a reliable, fast and accurate technique that allows the identification at species level of most microorganisms present in clinically relevant human samples. so it can be used in the daily routine of a clinical microbiology laboratory as an alternative or complement to traditional techniques. To validate this hypothesis, the initial objective of evaluating the MALDI-TOF VITEK MSTM system (bioMérieux, Marcy l'Etoile, France) was set out in a collection of strains that included a variety of species representative of those most frequently isolated in our laboratory. The application of the technique to resolve the species-level identification of 3 groups of microorganisms was then evaluated: Acinetobacter baumannii complex (ACB), Rhodococcus equi/Dietzia spp. and Candida spp.. Finally, conclude that MALDI-TOF technology is a good methodology for routine activity in clinical microbiology laboratories, identifying a wide range of clinically important bacterial and yeast species, quickly and accurately.