Study of human dental pulp stem cells (hDPSCs) combined with titanium, decellularized adipose tissue and plasma derived products on osteoinduction for bone tissue engineering

Abstract

We studied the human Dental Pulp Stem Cells (hDPSCs) adherence, proliferation, viability and osteo-differentiation potential when cultured on widely used Ti6AL4V titanium surface and a new biomimetic porous surface (BASTM), in the presence or absence of osteoblastic differentiation media supplemented with plasma rich in growth factors (PRGF) and platelet rich fibrin (PRF). Once we studied the effects of these two titanium surfaces on hDPSCs, we performed a comparative study of viability, proliferation and osteoblastic differentiation potential between the most promising mesenchymal stem cells for bone regeneration therapies, hDPSCs and hBMSCs. Lastly, another problem in dental implantology is the loss of periodontal ligament. The function of this tissue is to act like a cushion to absorb part of the masticatory mechanical forces to protect the alveolar bone. Nowadays, the periodontal regenerative therapy is based on the use of barrier membranes in alveolar ridge defects to enhance the bone growth on the surrounding affected area before the placement of dental implants. For this purpose, we studied the porcine decellularized adipose tissue (pDAT). // We studied the human Dental Pulp Stem Cells (hDPSCs) adherence, proliferation, viability and osteo-differentiation potential when cultured on widely used Ti6AL4V titanium surface and a new biomimetic porous surface (BASTM), in the presence or absence of osteoblastic differentiation media supplemented with plasma rich in growth factors (PRGF) and platelet rich fibrin (PRF). Once we studied the effects of these two titanium surfaces on hDPSCs, we performed a comparative study of viability, proliferation and osteoblastic differentiation potential between the most promising mesenchymal stem cells for bone regeneration therapies, hDPSCs and hBMSCs. Lastly, another problem in dental implantology is the loss of periodontal ligament. The function of this tissue is to act like a cushion to absorb part of the masticatory mechanical forces to protect the alveolar bone. Nowadays, the periodontal regenerative therapy is based on the use of barrier membranes in alveolar ridge defects to enhance the bone growth on the surrounding affected area before the placement of dental implants. For this purpose, we studied the porcine decellularized adipose tissue (pDAT).