Autologous dental pulp mesenchymal stem cells for inferior third molar post-extraction socket healinga split-mouth randomised clinical trial

  1. Luis Barbier Herrero 1
  2. Eva Ramos Alonso 2
  3. Josu Mendiola 3
  4. Olivia Rodriguez 3
  5. Gorka Santamaría Arrieta 4
  6. Joseba Andoni Santamaría Zuazua 1
  7. María Iciar Arteagoitia Calvo 4
  1. 1 MD, PhD, Professor, Cruces University Hospital, BioCruces Health Research Institute, University of the Basque Country
  2. 2 PharD, PhD, Pharmacist, BioCruces Heath Research Institute
  3. 3 MD, Neuroradiologist, Cruces University Hospital, BioCruces Heath Research Institute
  4. 4 MD, PhD, Associate Professor, University of the Basque Country, BioCruces Health Research Institute
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Revista:
Medicina oral, patología oral y cirugía bucal. Ed. inglesa

ISSN: 1698-6946

Año de publicación: 2018

Volumen: 23

Número: 4

Páginas: 14

Tipo: Artículo

DOI: 10.4317/MEDORAL.22466 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Medicina oral, patología oral y cirugía bucal. Ed. inglesa

Objetivos de desarrollo sostenible

Resumen

Since the discovery of adult mesenchymal stem cells extensive research has been conducted to determine their mechanisms of differentiation and effectiveness in cell therapy and regenerative medicine. To assess the efficacy of autologous dental pulp mesenchymal stem cells delivered in a collagen matrix for post-extraction socket healing, a single-centre, double-blind, randomised, split-mouth, controlled clinical trial was performed. Both impacted mandibular third molars were extracted from 32 patients. Dental pulp was collected and dissociated; the resulting cell suspension, obtained by centrifugation, was incorporated into a resorbable collagen matrix and implanted in 32 experimental post-extraction sockets. Collagen matrices alone were implanted in 32 contralateral, control post-extraction sockets. Two neuroradiologists independently assessed the extent of bone repair at 6 months after the extractions. Computed tomography (CT, Philips Brilliance) and an advanced display platform (IntelliSpace Portal) was used to record extraction socket density, expressed as Hounsfield units (HU) and height (mm) of the distal interdental bone septum of the second molar. Measurements at 6 months post-extraction were compared with measurements obtained immediately after extraction. Data were analysed with the statistical program STATA 14. Two patients dropped out of the study. The final sample consisted of 22 women and 8 men (mean age, 23 years; range: 18–30 years). Clinical, radiological, and surgical characteristics of impacted third molars of the control and experimental groups were homogeneous. Measurements obtained by the two neuroradiologists showed agreement. No significant differences were found in the extent of bone repair during analyses of density (p=0.4203 neuroradiologist 1; p=0.2525 neuroradiologist 2) or interdental septum height (p=0.2280 neuroradiologist 1; p=0.4784 neuroradiologist 2). In our clinical trial, we were unable to demonstrate that autologous dental pulp mesenchymal stem cells reduce socket bone resorption after inferior third molar extraction.

Referencias bibliográficas

  • Gronthos, S,Mankani, M,Brahim, J,Robey, PG,Shi, . (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 25. 13625
  • Avinash, K,Malaippan, S,Dooraiswamy, J. (2017). Methods of isolation and characterization of stem cells from different regions of oral cavity using markers: A systematic review. Int J Stem Cells. 10. 12-20
  • Graziano, A,d'Aquino, R,Laino, . (2008). Papaccio G. Dental Pulp stem cells: a promising tool for bone regeneration. Stem Cell Rev. 1. 21
  • Graziano, A,Carinci, F,Scolaro, S,d'Aquino, . (2013). Periodontal tissue generation using autologous dental ligament micro-grafts: case report with 6 months follow-up. Annals Oral Maxillofac Surg. 2. 20
  • Brunelli, G,Motroni, A,Graziano, A,D'Aquino, R,Zollino, I,Carence, . (2013). Sinus lift tissue engineering using autologous pulp micro-grafts: A case report of bone density evaluation. J Indian Soc Periodontol. 17. 644
  • Trovato, L,Monti, M,Del Fante, C,Cervio, M,Lampinen, M,Ambrosio, . (2015). A New Medical Device Rigeneracons Allows to Obtain Viable Micro-Grafts From Mechanical Disaggregation of Human Tissues. J Cell Physiol. 230. 2299
  • d'Aquino, R,De Rosa, A,Lanza, W,Trino, V,Laino, L,Graziano, . (2009). Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cells Mater. 18. 75-83
  • Giuliani, A,Manescu, A,Langer, M,Rustichelli, F,Desiderio, V,Paino, . (2013). Three years after transplants in human mandibles, histological and in-line holotomography revealed that stem cells regenerated a compact rather than a spongy bone: biological and clinical implications. Stem Cells Transl Med. 2. 316
  • d'Aquino, R,Trovato, L,Graziano, A,Ceccarelli, G,Cusella de Angelis, G,Marangini, . (2016). Periosteum-derived micro-grafts for tissue regeneration of human maxillary bone. J Transl Sci. 2. 125
  • Schropp, L,Wenzel, A,Kostopoulos, L,Karring, . (2003). Bone healing and soft tissue contour changes following single-tooth extraction: A clinical and radiographic 12-month prospective study. Int J Periodontics Restorative Dent. 23. 313
  • Arteagoitia, I,Ramos, E,Santamaria, G,Barbier, L,Alvarez, L,Santamaria, . (2015). Amoxicillin/Clavulanic acid 2000/125 mg to prevent complications due to infection following completely bone-impacted lower thrid molar removal: a clinical trial. Oral Sur, Oral Med, Oral Pathol, Oral Radiol. 119. 8-16
  • Cobos-Carbo, A,Agustovskt, . (2011). CONSORT 2010 Declaration: Updated guideline for reporting paralell group randomized trials. Med Clin. 137. 213
  • D'Aquino, R,Graziano, A,Sanpaolesi, M,Laino, G,Pirozzi, G,De Rosa, . (2007). Human postnatal dental pulp cells co-diferentiate into osteoblast endotheliocutes: a pivotal synergy leading to adult bone tissue formation. Cell Death Differ. 14. 1162
  • Graziano, A,d'Aquino, R,Laino, G,Giuliano, MT,Pirozzi, G,De Rosa, . (2008). Human CD34+ stem cells produce nodules in vivo. Cell Prolif. 41. 1-11
  • Stenderup, K,Justensen, J,Clausen, C,Kassen, . (2003). Aging is associated with decrease maximal life span and accelerated senescence of bone marow stromal cells. Bone. 33. 919
  • Leyendecker, A,Gomes, CC,Lazarretti, T,Franco, . (2018). The use of human dental pulp stem cells for in vivo tissue engineering: A systematic review. J Tissue Eng. 17.
Fuente de los datos: Dialnet