Estudio de dos pautas de inmunosupresión tras alotrasplante límbico

  1. I Rodríguez Agirretxe
  2. J Mendicute
  3. P Aldazábal
  4. N García Urquía
  5. J Durán de la Colina
Journal:
Archivos de la Sociedad Española de Oftalmologia

ISSN: 0365-6691

Year of publication: 2003

Volume: 78

Issue: 1

Pages: 29-34

Type: Article

DOI: 10.4321/S0365-66912003000100007 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Archivos de la Sociedad Española de Oftalmologia

Abstract

Purpose: Comparison of two immunosuppressive strategies after limbal allograft transplantation in an experimental model of ocular surface chemical burn. Methods: Twenty albino rabbits underwent corneal and limbal chemical burn with NaOH. One week later limbal transplantation was performed. Clinical assessments included measurements of epithelial defect, stromal opacity and neovascularization areas, using a computerized planimetry system 0, 7, 15, 30, 60 and 90 days after the chemical burn. Histologic examination was performed at 3 months, after sacrifice of the animals. Two immunosuppressive strategies were evaluated: topical cyclosporin A on constant dosage (group 1), and a combination of topical cyclosporin A and dexamethasone followed by a tapered dosage of topical cyclosporin A (group 2). Results: No statistical differences were observed between both groups regarding all of the variables 3 months after the chemical burn. Differences in the stromal opacity area were detected on day 7 (43.33 D.E. 4.84 vs 36.67 D.E. 3.88; p<0.01), day 15 (41.68 3 D.E..95 vs 33.6 D.E. 1.99; p<0.01), and day 30 (40.1 D.E. 5.45 vs 32.52 D.E. 7.33; p<0.05). As for the neovascularization area, differences were detected on day 7 (1.92 D.E. 0.48 vs 3.73 D.E. 1.18; p<0.01), day 15 (7.08 D.E. 1.54 vs 4.42 D.E. 1.68; p<0.01), and day 60 (4.9 D.E. 2.58 vs 9.65 D.E. 5.55; p<0.05). Conclusions: There are no long-term differences between both immunosuppressive strategies regarding all the studied variables.

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