Estrés oxidativo en el nervio óptico de la rata inducido por la administración crónica de etanol

  1. J. Aviñó 1
  2. Manuel Díaz Llopis 2
  3. Enrique España Gregori 1
  4. S. Johnsen Soriano 3
  5. B. Romero 4
  6. N. Marín
  7. María Muriach 3
  8. Francisco Bosch-Morell 3
  9. F.J. Romero 5
  1. 1 Hospital Universitari i Politècnic La Fe
  2. 2 Sociedad Española de Miopía
  3. 3 Fundación Oftalmológica del Mediterráneo, Valencia, Spain
  4. 4 Hospital San Rafael (Barcelona)
  5. 5 Universidad CEU Cardenal Herrera

    Universidad CEU Cardenal Herrera

    Valencia, España


Archivos de la Sociedad Española de Oftalmologia

ISSN: 0365-6691

Year of publication: 2002

Volume: 77

Issue: 5

Pages: 263-268

Type: Article

More publications in: Archivos de la Sociedad Española de Oftalmologia


Purpose To assess the involvement of oxidative stress in optic nerves after chronic intake of ethanol in adult rats, when compared to animals fed with an ethanol-free isocaloric diet. Methods Male Sprague-Dawley rats were used throughout the study. They were fed an ethanol-containing liquid diet, whereas the pair-fed group was given an ethanol-free isocaloric diet. After six weeks of the experiment, optic nerves were extracted and markers of oxidative stress were assayed, i.e., antioxidants such as glutathione and lipid peroxidation products such as malondialdehyde (MDA). Results The GSH content in the optic nerves of ethanol-fed animals was significantly reduced, and the concentration of MDA was significantly higher in this group when compared with the pair-fed group. Time-course of body weight of animals in both groups varied identically throughout the six weeks of the experiment. Conclusion The increase in lipid peroxidation products (MDA), together with the decrease in cellular antioxidants (GSH) confirm, in this experimental model, the involvement of oxidative stress in ethanol-induced toxicity of the optic nerves of rats. In view of the body weight time course, the influence of nutritional status on the parameters studied could also be discarded (Arch Soc Esp Oftalmol 2002; 77: 263-268).