NMR and molecular recognitionthe interaction of human galectin-8 and DC-SIGN with natural glycans and glycomimetics

Résumé

Glycans are everywhere in Nature, where they play a plethora of functions in every domain of life. The recognition of glycans by lectins is at the heart of a myriad of biological proceses, including host-pathogen interactions, which have made them essential targets in the field of drug discovery. In this context, the main focus of this Thesis has centred on unravelling of the conformational and structural properties of the recognition of a battery of natural and synthetically obtained glycans to two specific human lectins, galectin-8 and DC-SIGN. This analysis has been performed employing a synergic combination of NMR-based methods, assisted by other experimental methods (ITC) and computational tools (Molecular Dynamics simulations).